Curriculum Vitae Slides

Minh-Anh Huynh

Minh-Anh Huynh

26 years old

French nationality, Vietnamese origin,

  • Objective:
    Pharmacist-Researcher & Computational Immunology

  • Ph.D. in Bioinformatics

  • Post-doc in academy

Education

Doctor of Pharmacy (2017-2024)

  • Currently finishing my pharmaceutical thesis on Vitamin D and COVID-19 (expected in February 2024).

  • Basic R programming and statistics

  • Pharmacokinetics, pharmacology, genetics, chemistry (inorganic, organic, analytical)

Master’s Degree (2021-2022)

  • M1 Immunology University of Paris-Cité

    • General Microbiology, Immunology and Immunopathology.
  • M2: Systems Immunology (Molecular and Cellular Biology) at Sorbonne University

    • Conferences on systems biology, computational aspect and bioinformatics

    • Hands-on R programming workshops on downstream analysis of bulk RNA-Seq data

Publications

  • Gautier, Candice, Minh-Anh Huynh, Camille Peron, et Jonathan Pol. 2023. «Bacteria modified to produce L-arginine potentiate cancer immunotherapy». Médecine/Sciences 39 (10): 793‑95. https://doi.org/10.1051/medsci/2023109.

  • Minh-Anh Huynh, Agnès Ostertag, Camille Péron, Dominique Modrowski, Multilayered control of inflammation/immunity in osteosarcomas. Contribution of the sarcoma stem cells, Pasteur International Cancer Conference, 2023, Institut Pasteur, Paris, France

Multilayered control of inflammation/immunity in osteosarcomas. Contribution of the sarcoma stem cells

Internships

Bioinformatics internship at Lariboisière (INSERM) | (2022) 4 months

Determination of inflammatory status in osteosarcoma samples and association with osteosarcoma phenotypes, mechanisms and pathways

  • R programming
  • Construction of a custom inflammatory index based on hallmark MSigDB inflammatory signature
  • Bulk RNA-Seq
  • Differential gene expression analysis (DESeq2)
  • Functional Pathway Analysis (enrichR)
  • GSEA
  • Tools: MDS, heatmaps, UMAP, k-means clustering, survival plot, MCP counter (immune deconvolution), ESTIMATE score, statistical analysis, Bioconductor Github
  • Not enough data for scRNA-Seq (tentative nichenet project)

Heatmap of Hallmark Inflammatory Response Signature from MSigDB

Neuroinflammation internship | 2021 (4 months)

  • Study of microglial activation in a mouse model of comorbid cocaine addiction and post-traumatic stress.
  • Immunohistochemistry manipulation, quantification and creation of macros using Image J and Pulover.

Bibliographic | 2 months

  • Internship on Spike-ACE2 interactions of SARS-CoV-2 and pathophysiology of COVID-19.

Industry Track Group Project

Camostat Mesylate in COVID-19 | 2020-2021 (6 months)

  • Management of the clinical division and clinical trial design.

  • Pharmacokinetics of Camostat, bioavailability in lungs.

Research ideas about Ph.D.

Vitamin D and COVID-19

  • Currently working on pharmacy thesis about Vitamin D and COVID-19

  • Both the incidence and severity of COVID-19 are epidemiologically associated with VitD deficiency/insufficiency

  • Strong relationship between immune tolerance and (insufficient) vitamin D levels: ubiquitous VDR + key cytokines and transcription factors

  • Vitamin D surprisingly plays many roles:

    • cardiovascular system (thrombotic events);

    • Immune tolerance and immune health;

    • Renin Angiotensin System (lung injury, cardiovascular, inflammation);

    • Protection against acute respiratory lung infections;

      • Implication: Vitamin D reshapes the epigenetic landscape of (immune) cells (Chauss et al., 2022)
  • New research literature since 2011 (IOM) show that there might be a need to increase VitD levels (from 20-30 ng/ml) to at least 40 ng/mL for immunological benefits

Chauss et al. (2022) : Investigated molecular mechanisms of CD4+ responses in COVID-19

VitD induces IL-10 in CD4+
  • Use spatial transcriptomics to investigate and identify lymphocytes types/states in relation to inflammation?

Depression and inflammation

Metabolomic analysis of treatment-refractory major depressive disorder with suicidal ideation. Pan et al. (2023)

Role of vitamin D ?

Spatial Transcriptomics (ST) is a powerful technology to map the molecular space in 2D. Our lab is using a wide range of ST technologies to investigate how drugs are delivered to various organs including tumor mass at the single cell resolution.

We are looking for excellent candidates willing to investigate various in vivo drug delivery vehicles to learn latest bioinformatics tools to develop efficient and cell-specific drug delivery tools.

References

Chauss,D. et al. (2022) Autocrine vitamin D signaling switches off pro-inflammatory programs of TH1 cells. Nature Immunology, 23, 62–74.
Cowen,P.J. and Browning,M. (2015) What has serotonin to do with depression? World Psychiatry, 14, 158–160.
Kubera,M. et al. (2011) In animal models, psychosocial stress-induced (neuro)inflammation, apoptosis and reduced neurogenesis are associated to the onset of depression. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 35, 744–759.
Lacasse,J.R. and Leo,J. (2005) Serotonin and Depression: A Disconnect between the Advertisements and the Scientific Literature. PLoS Medicine, 2, e392.
Liu,Y.-Z. et al. (2017) Inflammation: The Common Pathway of Stress-Related Diseases. Frontiers in Human Neuroscience, 11, 316.
Moncrieff,J. et al. (2022) The serotonin theory of depression: a systematic umbrella review of the evidence. Molecular Psychiatry, 1–14.
Pan,L.A. et al. (2023) Metabolic features of treatment-refractory major depressive disorder with suicidal ideation. Translational Psychiatry, 13, 393.
Taler,M. et al. (2007) Immunomodulatory effect of selective serotonin reuptake inhibitors (SSRIs) on human T lymphocyte function and gene expression. European Neuropsychopharmacology, 17, 774–780.