Doctor of Pharmacy (2017-2024)

• Currently finishing my pharmaceutical thesis on Vitamin D and COVID-19 (expected in February 2024).

• Basic R programming and statistics

• Pharmacokinetics, pharmacology, genetics, chemistry (inorganic, organic, analytical)

Master’s Degree (2021-2022)

• M1 Immunology University of Paris-Cité

  • General Microbiology, Immunology and Immunopathology.

• M2: Systems Immunology (Molecular and Cellular Biology) at Sorbonne University

  • Conferences on systems biology, computational aspect and bioinformatics

  • Hands-on R programming workshops on downstream analysis of bulk RNA-Seq data

Publications

• Gautier, Candice, Minh-Anh Huynh, Camille Peron, et Jonathan Pol. 2023. «Bacteria modified to produce L-arginine potentiate cancer immunotherapy». Médecine/Sciences 39 (10): 793‑95. https://doi.org/10.1051/medsci/2023109.

• Minh-Anh Huynh, Agnès Ostertag, Camille Péron, Dominique Modrowski, Multilayered control of inflammation/immunity in osteosarcomas. Contribution of the sarcoma stem cells, Pasteur International Cancer Conference, 2023, Institut Pasteur, Paris, France

Bioinformatics internship at Lariboisière (INSERM) | (2022) 4 months

Determination of inflammatory status in osteosarcoma samples and association with osteosarcoma phenotypes, mechanisms and pathways

• R programming
• Construction of a custom inflammatory index based on hallmark MSigDB inflammatory signature
• Bulk RNA-Seq
• Differential gene expression analysis (DESeq2)
• Functional Pathway Analysis (enrichR)
• GSEA
• Tools: MDS, heatmaps, UMAP, k-means clustering, survival plot, MCP counter (immune deconvolution), ESTIMATE score, statistical analysis, Bioconductor Github
• Not enough data for scRNA-Seq (tentative nichenet project)

Neuroinflammation internship | 2021 (4 months)

• Study of microglial activation in a mouse model of comorbid cocaine addiction and post-traumatic stress.
• Immunohistochemistry manipulation, quantification and creation of macros using Image J and Pulover.

Bibliographic | 2 months

• Internship on Spike-ACE2 interactions of SARS-CoV-2 and pathophysiology of COVID-19.

Industry Track Group Project

Camostat Mesylate in COVID-19 | 2020-2021 (6 months)

• Management of the clinical division and clinical trial design.

• Pharmacokinetics of Camostat, bioavailability in lungs.

Vitamin D and COVID-19

• Currently working on pharmacy thesis about Vitamin D and COVID-19

• Both the incidence and severity of COVID-19 are epidemiologically associated with VitD deficiency/insufficiency

• Strong relationship between immune tolerance and (insufficient) vitamin D levels: ubiquitous VDR + key cytokines and transcription factors

• Vitamin D surprisingly plays many roles:

  • cardiovascular system (thrombotic events);

  • Immune tolerance and immune health;

  • Renin Angiotensin System (lung injury, cardiovascular, inflammation);

  • Protection against acute respiratory lung infections;

    • Implication: Vitamin D reshapes the epigenetic landscape of (immune) cells (Chauss et al., 2022)

• New research literature since 2011 (IOM) show that there might be a need to increase VitD levels (from 20-30 ng/ml) to at least 40 ng/mL for immunological benefits

Further explore links between VitD and COVID-19 through bioinformatical approach

Chauss et al. (2022) : Investigated molecular mechanisms of CD4⁺ responses in COVID-19

• Use spatial transcriptomics to investigate and identify lymphocytes types/states in relation to inflammation?

Depression and inflammation

• Literature research state serotonin theory has nothing to depression (Lacasse and Leo, 2005; Cowen and Browning, 2015 ; Moncrieff et al., 2022).

• Hypothesis of depression linked to elevated brain inflammation (Liu et al., 2017 ; Kubera et al., 2011)

• SSRI are actually anti-inflammatory (Taler et al., 2007)

• Brain: Examining relationship between elevated brain inflammation and depression disorders with possible links to treatment resistant depression, on an individualized level

Role of vitamin D ?